Assalamualaikum, and good day to my Histo Tech students. Lectures 2, 3 & 4 can be downloaded here, here and here. Don’t forget to say thanks okayyyy..Below are NOT the slideshow of full lecture notes but only the front pages of each, coz I don’t really have much time to upload every single slide.
Briefly, the tissue processing notes explain about the subsequent procedures after fixation that must be done in order to remove water from tissues and to replace it with a hard medium like paraffin wax. The steps involved are dehydration, clearing, infiltration and impregnation and finally embedding. Remember?
And for sectioning, still remember what equipment that we used? Well it’s called a microtome. As I told you, we use this equipment to cut thinly those tissue blocks that have been prepared during embedding process, which will result in the production of ribbons or sections. And please don’t forget about the ‘problems, causes and remedies’ part bcoz the chance for any of those to be come out in the exam is pretty high. okay?
Finally, I haven’t yet teach you about the staining topic but you may study it yourself first. Briefly this lecture would tell you the theory of staining, the characteristics of several dyes commonly used in staining as well as the most commonly used staining method in a Histology lab, which is the Haematoxylin and Eosin staining (H&E). Included also the outlines and actual protocol of the technique.
Never mind, if you don’t understand you may ask me in our next meeting okay. TQ..